TAT-BINDING PROTEIN-7 IS A SUBUNIT OF THE 26S PROTEASE

被引：42

作者：

DUBIEL, W ^{[1
]}

FERRELL, K ^{[1
]}

RECHSTEINER, M ^{[1
]}

机构：

[1] UNIV UTAH,SCH MED,DEPT BIOCHEM,SALT LAKE CITY,UT 84132

来源：

BIOLOGICAL CHEMISTRY HOPPE-SEYLER | 1994年 / 375卷 / 04期

关键词：

HUMAN RED BLOOD CELLS; TAT-BINDING PROTEIN; UBIQUITIN; ATP-DEPENDENT 26S PROTEASE;

D O I：

10.1515/bchm3.1994.375.4.237

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Subunit 6 (S6), an integral component of the 26S protease from human erythrocytes, has been studied by SDS-PAGE, peptide mapping and sequence analysis. S6 was cleaved with CNBr and three internal peptides were sequenced. A comparison with known proteins in Genbank revealed that all three S6 peptides match the predicted sequence of TBP7, Tat-binding protein 7. Based on peptide matches covering more than 10% of the TBP7 sequence, and the fact that the migration of S6 on SDS-PAGE is consistent with the estimated molecular mass for TBP7, we conclude that subunit 6 of the 26S protease is TBP7.

引用

页码：237 / 240

页数：4

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