IDENTIFICATION OF THE RON GENE-PRODUCT AS THE RECEPTOR FOR THE HUMAN MACROPHAGE STIMULATING PROTEIN

被引：240

作者：

WANG, MH

RONSIN, C

GESNEL, MC

COUPEY, L

SKEEL, A

LEONARD, EJ

BREATHNACH, R

机构：

[1] CHR NANTES,INST BIOL,INSERM,U211,F-44035 NANTES 01,FRANCE

[2] FAC MED LIMOGES,HEMATOL EXPTL LAB,F-87025 LIMOGES,FRANCE

来源：

SCIENCE | 1994年 / 266卷 / 5182期

关键词：

D O I：

10.1126/science.7939629

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Macrophage-stimulating protein (MSP) is a member of the hepatocyte growth factor-scatter factor (HGF-SF) family. Labeled MSP bound to Madin-Darby canine kidney (MDCK) cells transfected with complementary DNA encoding Ron, a cell membrane protein tyrosine kinase. Cross-lin king of I-125-labeled MSP to transfected cells (MDCK-RE7 cells) and immunoprecipitation by antibodies to Ron revealed a 220-kilodalton complex, a size consistent with that of MSP (80 kilodaltons) cross-linked to the beta chain of Ron (150 kilodaltons). The binding of I-125-labeled MSP to MDCK-RE7 cells was inhibited by unlabeled MSP, but not by HGF-SF. MSP caused phosphorylation of the beta chain of Ron and induced migration of MDCK-RE7 cells. These results establish the ron gene product as a specific cell-surface receptor tor MSP.

引用

页码：117 / 119

页数：3

共 25 条

[21]

WANG MH, 1994, J BIOL CHEM, V269, P13806

[22]

WANG MH, 1994, J BIOL CHEM, V269, P3436

[23] EVIDENCE FOR THE IDENTITY OF HUMAN SCATTER FACTOR AND HUMAN HEPATOCYTE GROWTH-FACTOR [J].