DUAL-SPECIFICITY INTERACTION OF HIV-1 TAR RNA WITH TAT PEPTIDE-OLIGONUCLEOTIDE CONJUGATES

被引：32

作者：

TUNG, CH

WANG, JH

LEIBOWITZ, MJ

STEIN, S

机构：

[1] CABM,PISCATAWAY,NJ 08054

[2] RUTGERS STATE UNIV,DEPT CHEM,PISCATAWAY,NJ 08855

[3] UNIV MED & DENT NEW JERSEY,ROBERT WOOD JOHNSON MED SCH,DEPT MOLEC GENET & MICROBIOL,PISCATAWAY,NJ 08854

来源：

BIOCONJUGATE CHEMISTRY | 1995年 / 6卷 / 03期

关键词：

D O I：

10.1021/bc00033a009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An oligonucleotide-peptide conjugate, having dual binding capability for a designated RNA, was designed. The peptide portion of the conjugate interacts with a folded domain in the RNA, whereas the oligonucleotide portion hybridizes with a nearby single-stranded region in the RNA. The dual specificity was proven in a model HIV-1 TAR RNA system using an RNase H cleavage assay to assess antisense binding to this RNA. The peptide portion of the conjugate was shown to confer increased specificity on the oligonucleotide.

引用

页码：292 / 295

页数：4

共 25 条

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