IMMUNOLOGICALLY UNRELATED HELIOTHIS SP AND SPODOPTERA SP MIDGUT MEMBRANE-PROTEINS BIND BACILLUS-THURINGIENSIS CRYIA(B) DELTA-ENDOTOXIN

被引:50
作者
ODDOU, P [1 ]
HARTMANN, H [1 ]
RADECKE, F [1 ]
GEISER, M [1 ]
机构
[1] CIBA GEIGY AG, BIOTECHNOL PLANT PROTECT SECT, DIV AGRO, CH-4002 BASEL, SWITZERLAND
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 212卷 / 01期
关键词
D O I
10.1111/j.1432-1033.1993.tb17644.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Bacillus thuringiensis toxins, CryIA(a), CryIA(b), CryIA(c) and CryIC were used in a ligand-blot assay to detect specific toxin-binding proteins in the brush-border membranes of Heliothis virescens, Helicoverpa zea, Spodoptera littoralis, Spodoptera exigua and Spodoptera litura. While CryIA(a) and CryIA(b) always recognize the same protein(s) in a given species, CryIA(c) and CryIC were found to bind to other proteins. Polyclonal antibodies directed against the CryIA(b) binding protein of H. virescens and polyclonal anti-idiotype antibodies recognizing some determinants on the CryIA(b)-binding protein involved in the interaction with the toxin, were used to analyse immunological relationships among the toxin-binding proteins. The results showed that the 170-kDa toxin-binding proteins from the H. virescens and H. zea are immunologically related. However, the toxin-binding proteins from the Spodoptera species did not cross-react with either type of antibodies. Therefore, we conclude that the CryIA(b) toxin has different binding determinants on the toxin molecule itself which can interact with specific binding sites on the toxin-binding proteins from Heliothis sp. and Spodoptera sp.
引用
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页码:145 / 150
页数:6
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