STRUCTURAL-ANALYSIS BY CIRCULAR-DICHROISM OF SOME ENZYMES INVOLVED IN PLANT-CELL WALL DEGRADATION

被引：11

作者：

GOODENOUGH, PW

CLARK, DC

DURRANT, AJ

GILBERT, HJ

HAZLEWOOD, GP

WAKSMAN, G

机构：

[1] INST FOOD RES, DEPT FOOD STRUCT BIOPOLYMER TECHNOL, NORWICH LAB, NORWICH NR4 7UA, NORFOLK, ENGLAND

[2] UNIV NEWCASTLE UPON TYNE, DEPT AGR BIOCHEM & TUTR, NEWCASTLE UPON TYNE NE1 7RU, TYNE & WEAR, ENGLAND

[3] UNIV BRISTOL, DEPT BIOCHEM, CAMBRIDGE RES STN, BRISTOL BS8 1TH, AVON, ENGLAND

[4] UNIV BRISTOL, INST ANIM PHYSIOL & GENET RES, BRISTOL BS8 1TH, AVON, ENGLAND

[5] UNIV BRISTOL, DEPT AGR SCI, BRISTOL BS8 1TH, AVON, ENGLAND

来源：

FEBS LETTERS | 1991年 / 282卷 / 02期

关键词：

CELLULASE; POLYGALACTURONASE; XYLANASE;

D O I：

10.1016/0014-5793(91)80512-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Three enzymes which degrade different polysaccharide components of plant cell walls have been characterized by circular dichroism (CD). A bacterial endoglucanase, which in the native state forms part of a multiprotein cellulase complex, showed a tendency to form aggregates as measured by CD. Depending on its degree of aggregation, this enzyme displayed between 50% and 100% helical structure, whereas a bacterial xylanase and a fungal polygalacturonase exhibited more beta-sheet structure. the polygalacturonase was apparently devoid of helical structure.

引用

页码：355 / 358

页数：4

共 23 条

[1] CONFORMATION AND AGGREGATION OF MELITTIN - DEPENDENCE ON PH AND CONCENTRATION [J].