INVESTIGATION OF THE UPTAKE OF DRUGS, CARCINOGENS AND MUTAGENS BY INDIVIDUAL MAMMALIAN-CELLS USING A SCANNING PROTON MICROPROBE

被引：14

作者：

CHOLEWA, M

TURNBULL, IF

LEGGE, GJF

WEIGOLD, H

MARCUCCIO, SM

HOLAN, G

TOMLINSON, E

WRIGHT, PJ

DILLON, CT

LAY, PA

BONIN, AM

机构：

[1] CSIRO,DIV CHEM & POLYMERS,CLAYTON,VIC 3169,AUSTRALIA

[2] MONASH UNIV,DEPT MICROBIOL,CLAYTON,VIC 3168,AUSTRALIA

[3] UNIV SYDNEY,SCH CHEM,DIV INORGAN CHEM,SYDNEY,NSW 2006,AUSTRALIA

[4] NIOSH,SYDNEY,NSW,AUSTRALIA

[5] INST PHYS NUCL LYON,KRAKOW,POLAND

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 1995年 / 104卷 / 1-4期

基金：

澳大利亚研究理事会;

关键词：

D O I：

10.1016/0168-583X(95)00421-1

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The use of micro-PIXE [1] in measuring the quantitative uptake of drugs containing metal atoms by individual Vero cells (African green monkey kidney cell line) and V79 Chinese hamster lung cells is demonstrated. One class of drugs, heteropolytungstates, which are being assessed for activity against the HIV virus, were studied using Vero cells. The cellular uptake of a series of chromium compounds, including carcinogens and mutagens, in which the metal oxidation state was either (III), (V) or (VI), was measured using V79 cells. It was found that, unlike any other techniques, scanning proton microprobe (SPM) offers both the sensitivity and spatial resolution to carry out unicellular analysis. The use of cultured cell lines in these analyses was shown to have distinct advantages over cells such as peripheral blood lymphocytes (PBLs).

引用

页码：317 / 323

页数：7

共 11 条

[1] RECENT ADVANCES IN CHROMIUM GENOTOXICITY [J].