HEPATIC-FIBROSIS PRODUCED IN GUINEA-PIGS BY CHRONIC ETHANOL ADMINISTRATION AND IMMUNIZATION WITH ACETALDEHYDE ADDUCTS

被引：55

作者：

YOKOYAMA, H ^{[1
]}

NAGATA, S ^{[1
]}

MORIYA, S ^{[1
]}

KATO, S ^{[1
]}

ITO, T ^{[1
]}

KAMEGAYA, K ^{[1
]}

ISHII, H ^{[1
]}

机构：

[1] KEIO UNIV,SCH MED,DEPT INTERNAL MED,SHINJUKU KU,TOKYO 160,JAPAN

来源：

HEPATOLOGY | 1995年 / 21卷 / 05期

关键词：

D O I：

10.1016/0270-9139(95)90067-5

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Experimental hepatic fibrosis was produced in the guinea pig. We produced hepatic necrosis associated with inflammatory cell infiltration in guinea pigs immunized with acetaldehyde adducts and fed ethanol for 40 days. Extending the period of these treatments to 90 days resulted in producing hepatic fibrosis developing around individual hepatocytes in the terminal hepatic venule ar eas and portal areas, accompanied by an increase in hepatic hydroxyproline content. In contrast, no fibrosis was observed in the livers of the control groups that had been exposed to nothing, ethanol alone, or a combination of ethanol and immunization with unmodified human hemoglobin. Minimal fibrotic changes were observed in animals immunized with human hemoglobin acetaldehyde adducts but not fed ethanol. These results indicate that the formation of acetaldehyde adducts and the acquisition of immunity against them can produce hepatic fibrosis. Immune mechanisms against acetaldehyde adducts may, in part, be involved in the pathogenesis of hepatic fibrosis seen in alcoholics.

引用

页码：1438 / 1442

页数：5

共 24 条

[11]

Israel, Hurwitz, Niemela, Arnon, Monoclonal and polyclonal antibodies against acetaldehyde-containing epitopes in acetaldehyde-protein adduct, Proc Natl Acad Sci U S A, 83, pp. 7923-7927, (1986)

[12]

Niemela, Klajner, Orrego, Vidins, Blendis Israel, Antibodies against acetaldehyde-modified protein epitopes in human alcoholics, Hepatology, 7, pp. 1210-1214, (1987)

[13]

Hoerner, Behrens, Worner, Blacksberg, Braley, Schaffner, Lieber, The role of alcoholism and liver disease in the appearance of serum antibodies against acetaldehyde adducts, Hepatology, 8, pp. 569-574, (1988)

[14]

Yokoyama, Ishii, Nagata, Kato, Tsuchiya, Evidence for acetaldehyde adducts formation in hepatic microsomes and cytosol of guinea pig after chronic ethanol administration, Res Commun Subst Abuse, 12, pp. 173-180, (1991)

[15]

Yokoyama, Ishii, Nagata, Moriya, Ito, Kato, Tsuchiya, Heterogeneity of hepatic acetaldehyde adducts after chronic ethanol consumption: an immunohistochemical analysis with monoclonal and polyclonal antibodies against acetaldehyde containing epitopes in acetaldehyde adducts, Alcohol Alcohol, 28, pp. 91-97, (1993)

[16]

Yokoyama, Ishii, Nagata, Kato, Kamegaya, Tsuchiya, Experimental hepatitis induced by ethanol after immunization with acetaldehyde adducts, Hepatology, 17, pp. 14-19, (1993)

[17]

Yokoyama, Ishii, Nagata, Hibi, Tsuchimoto, Takagi, Maruyama, Et al., Antibodies against acetaldehyde adducts in alcoholics, Biomedical and Social Aspects of Alcohol and Alcoholism, pp. 537-539, (1988)

[18]

Inayama, Shibata, A new microanalytical method for detection of hydroxyproline in connective tissue, Keio J Med, 27, pp. 43-46, (1978)

[19]

Blackwell, Cirrhosis resulting from repeated injection of antigen, J Pathol Bacteriol, 90, pp. 245-257, (1965)

[20]

Yokoi, Namihisa, Matsuzaki, Miyazaki, Yamaguchi, Distribution of Ito cells in experimental hepatic fibrosis, Liver, 8, pp. 48-52, (1988)

← 1 2 3 →