ENZYMATIC-REACTION KINETIC - COMPARISON IN AN ORGANIC-SOLVENT AND IN SUPERCRITICAL CARBON-DIOXIDE

被引：48

作者：

DUMONT, T

BARTH, D

CORBIER, C

BRANLANT, G

PERRUT, M

机构：

[1] ECOLE NATL SUPER IND CHIM,PHYSICOCHIM IND LAB,1 RUE GRANDVILLE,F-54001 NANCY,FRANCE

[2] UNIV NANCY 1,ENZYMOL & GENIE GENET LAB,CNRS,UA 457,F-54506 VANDOEUVRE NANCY,FRANCE

[3] SOC SEPAREX,F-54250 CHAMPIGNEULLES,FRANCE

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1992年 / 40卷 / 02期

关键词：

LIPASE; SUPERCRITICAL CARBON DIOXIDE; KINETICS; ESTERIFICATION;

D O I：

10.1002/bit.260400218

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Myristic acid esterification has been performed by an immobilized lipase from Mucor Miehei both in n-hexane and in supercritical carbon dioxide (SCCO2). The enzyme is stable in SCCO2 at 15 MPa and 323 K. The reaction rate is influenced by the concentration of water and by the reaction medium composition. A reaction mechanism is proposed, and kinetic parameters are determined at 12.5 MPa and 313 K. Maximum velocity appears 1.5-fold higher in SCCO2 than in n-hexane; however, as solubility of myristic acid is greater in n-hexane, it is not yet definitively clear that the supercritical medium is more favorable than the classical organic solvent for this type of enzyme reaction.

引用

页码：329 / 333

页数：5

共 28 条

[1] MEASUREMENT AND MODEL PREDICTION OF SOLUBILITIES OF PURE FATTY-ACIDS, PURE TRIGLYCERIDES, AND MIXTURES OF TRIGLYCERIDES IN SUPERCRITICAL CARBON-DIOXIDE [J].