ANALYSIS OF OPERONS ENCODING 23S RIBOSOMAL-RNA OF CLOSTRIDIUM-BOTULINUM TYPE-A

被引：12

作者：

EAST, AK ^{[1
]}

THOMPSON, DE ^{[1
]}

COLLINS, MD ^{[1
]}

机构：

[1] INST FOOD RES, DEPT MICROBIOL, READING LAB, READING RG6 2EF, ENGLAND

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 24期

关键词：

D O I：

10.1128/JB.174.24.8158-8162.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Southern hybridization analysis of Clostridium botulinum type A chromosomal DNA indicated the presence of six copies of the 23S rRNA gene. Fragments of DNA encoding 23S rRNA were amplified by polymerase chain reaction and cloned in Escherichia coli. Three clones examined by restriction enzyme and sequence analysis were found to be derived from different operons. Sequence determination of the entire insert of two clones revealed nine nucleotide changes in the genes coding for 23S rRNA (99.7% sequence identity) between operons encoded on the same chromosome, showing microheterogeneity in the rRNA operons of this organism.

引用

页码：8158 / 8162

页数：5

共 27 条

[1]

ASH C, 1992, FEMS MICROBIOL LETT, V94, P75

[2] NOVEL TRANSFER-RNA GENE ORGANIZATION IN THE 16S-23S INTERGENIC SPACER OF THE STREPTOCOCCUS-PNEUMONIAE RIBOSOMAL-RNA GENE-CLUSTER [J].

BACOT, CM ;

REEVES, RH .

JOURNAL OF BACTERIOLOGY, 1991, 173 (13) :4234-4236

[3] TRANSCRIPTIONAL ANALYSIS OF THE 16S RIBOSOMAL-RNA GENE OF THE RRND GENE SET OF STREPTOMYCES-COELICOLOR A3(2) [J].

BAYLIS, HA ;

BIBB, MJ .

MOLECULAR MICROBIOLOGY, 1988, 2 (05) :569-579

[4] COMPLETE NUCLEOTIDE-SEQUENCE OF THE RIBOSOMAL 16-S-RNA FROM ESCHERICHIA-COLI - EXPERIMENTAL DETAILS AND CISTRON HETEROGENEITIES [J].

CARBON, P ;

EHRESMANN, C ;

EHRESMANN, B ;

EBEL, JP .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1979, 100 (02) :399-410

[5]

Cato E. P., 1986, BERGEYS MANUAL SYSTE, P1141

[6]

EAST AK, 1992, FEMS MICROBIOL LETT, V95, P57

[7]

FARROW JAE, 1983, J GEN MICROBIOL, V129, P1423

[8] ORGANIZATION AND NUCLEOTIDE-SEQUENCE ANALYSIS OF AN RIBOSOMAL-RNA AND TRANSFER-RNA GENE-CLUSTER FROM CAULOBACTER-CRESCENTUS [J].

FEINGOLD, J ;

BELLOFATTO, V ;

SHAPIRO, L ;

AMEMIYA, K .

JOURNAL OF BACTERIOLOGY, 1985, 163 (01) :155-166

[9] CLONING, MAPPING, AND MOLECULAR CHARACTERIZATION OF THE RIBOSOMAL-RNA OPERONS OF CLOSTRIDIUM-PERFRINGENS [J].

GARNIER, T ;

CANARD, B ;

COLE, ST .

JOURNAL OF BACTERIOLOGY, 1991, 173 (17) :5431-5438

[10] IDENTIFICATION OF CLOSTRIDIUM-BOTULINUM, CLOSTRIDIUM-ARGENTINENSE, AND RELATED ORGANISMS BY CELLULAR FATTY-ACID ANALYSIS [J].

GHANEM, FM ;

RIDPATH, AC ;

MOORE, WEC ;

MOORE, LVH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (06) :1114-1124

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