CORTICOSTEROIDOGENESIS IN THE TOAD BUFO-ARENARUM H - EVIDENCE FOR A PRECURSOR ROLE FOR AN ALDOSTERONE 3-BETA-HYDROXY-5-ENE ANALOG (3-BETA, 11-BETA, 21-TRIHYDROXY-20-OXO-5-PREGNEN-18-AL)

被引：8

作者：

CEBALLOS, NR

SHACKLETON, CH

HARNIK, M

COZZA, EN

GROS, EG

LANTOS, CP

机构：

[1] CHILDRENS HOSP,OAKLAND RES INST,OAKLAND,CA 94609

[2] UNIV BUENOS AIRES,CONICET,PRHOM,RA-1428 BUENOS AIRES,ARGENTINA

[3] UNIV BUENOS AIRES,DEPT QUIM BIOL,ESTEROIDES LAB,RA-1428 BUENOS AIRES,ARGENTINA

[4] TEL AVIV UNIV,GEORGE WISE FAC LIFE SCI,DEPT BIOTECHNOL & MICROBIOL,IL-69978 TEL AVIV,ISRAEL

[5] UNIV BUENOS AIRES,DEPT QUIM ORGAN,RA-1428 BUENOS AIRES,ARGENTINA

来源：

BIOCHEMICAL JOURNAL | 1993年 / 292卷

关键词：

D O I：

10.1042/bj2920143

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A material isolated following pregnenolone incubations with toad (Bufo arenarum) inter-renal tissue at 28-degrees-C has been identified as a 3beta-hydroxy-5-ene analogue of aldosterone (3,8,11,6,21-trihydroxy-20-oxo-5-pregnen-18-al). The initial identification was made by enzymic and m.s. methods, and structural confirmation was achieved through comparison with chemically synthesized authentic material. The relative efficacy of corticosterone, 18-hydroxycorticosterone and the 3beta-hydroxy-5-ene aldosterone analogue as aldosterone precursors was evaluated. In the in vitro situation studied, the 3beta-hydroxy-5-ene steroid was by far the best precursor.

引用

页码：143 / 147

页数：5

共 14 条

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