HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF CARNOSIC ACID AND CARNOSOL IN ROSMARINUS-OFFICINALIS AND SALVIA-OFFICINALIS

被引：109

作者：

OKAMURA, N

FUJIMOTO, Y

KUWABARA, S

YAGI, A

机构：

[1] Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Hiroshima

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 679卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)80582-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic method for the determination of carnosic acid and carnosol, phenolic diterpenes, which showed high antioxidative effect, is established. The analysis can be accomplished within 15 min under isocratic conditions with 0.1% phosphoric acid-60% acetonitrile as the mobile phase at a flow-rate of 1.0 ml/min, with detection at 230 nm. The detection limits of carnosic acid and carnosol are 0.104 and 0.521 ng per injection, respectively. This experimental system permits a good separation and quantification of these phenolic diterpenes in the leaves of Rosmarinus officinalis L. and Salvia officinalis L.

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页码：381 / 386

页数：6

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