MODULATORY EFFECT OF CROCETIN ON AFLATOXIN-B1 CYTOTOXICITY AND DNA ADDUCT FORMATION IN C3H10T1/2 FIBROBLAST CELL

被引：29

作者：

WANG, CJ ^{[1
]}

SHIAH, HS ^{[1
]}

LIN, JK ^{[1
]}

机构：

[1] NATL TAIWAN UNIV,COLL MED,INST BIOCHEM,TAIPEI,TAIWAN

来源：

CANCER LETTERS | 1991年 / 56卷 / 01期

关键词：

CROCETIN; AFLATOXIN-B1; CYTOTOXICITY; GLUTATHIONE; GLUTATHIONE S-TRANSFERASE; GLUTATHIONE PEROXIDASE; AFLATOXIN B1-DNA ADDUCT;

D O I：

10.1016/0304-3835(91)90186-L

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Crocetin is a carotenoid isolated from the seeds of Cape jasmine (Gardenia jasminoides). The cytotoxicity and DNA-adduct formation of rat microsome-activated aflatoxin B1 (AFB1) in the C3H10T1/2 cells were significantly inhibited by pretreatment of crocetin. Most significant inhibition was found at the time of 9 h after crocetin pretreatment. Under these experimental conditions, consistent elevation in the cytosolic glutathione (GSH) levels and the activities of GSH S-transferase (GST) and GSH-peroxidase (GSH-Px) were observed. Crocetin treatment also resulted in a decrease in AFB1-DNA adduct formation in vitro, while no effect of crocetin on the formation of AFB1-8,9-oxide in vitro system was detected as measured by the Trisdiol method. From these results, we suggested that the protective effect of crocetin on the AFB1-cytotoxicity in C3H10T1/2 cells might be due to the cellular defense mechanisms that elevated the cytosol GSH and the activities of GST and GSH-Px.

引用

页码：1 / 10

页数：10

共 38 条

[1] METHODS FOR DETECTING CARCINOGENS AND MUTAGENS WITH SALMONELLA-MAMMALIAN-MICROSOME MUTAGENICITY TEST [J].