LOCALIZATION OF TRANSFORMING GROWTH FACTOR-BETA-1 IN MITOCHONDRIA OF MURINE HEART AND LIVER

被引：75

作者：

HEINE, UI ^{[1
]}

BURMESTER, JK ^{[1
]}

FLANDERS, KC ^{[1
]}

DANIELPOUR, D ^{[1
]}

MUNOZ, EF ^{[1
]}

ROBERTS, AB ^{[1
]}

SPORN, MB ^{[1
]}

机构：

[1] PROGRAM RESOURCES INC DYNCORP, FREDERICK CANC RES & DEV CTR, FREDERICK, MD 21702 USA

来源：

CELL REGULATION | 1991年 / 2卷 / 06期

关键词：

D O I：

10.1091/mbc.2.6.467

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Using both electron microscopic immunohistochemistry and cell fractionation techniques, we show that transforming growth factor-beta-1 (TGF-beta-1) is found in mitochondria of rat and mouse cardiac myocytes and rat hepatocytes. Four different polyclonal antibodies, raised against various epitopes encompassing the mature portion of the TGF-beta-1 molecule as well as the pro-region of its precursor, were used for the electron microscopy studies. The localization of TGF-beta-1 in mitochondria was confirmed by detection of the native peptide in mitochondria isolated from rat heart and liver; the majority of native TGF-beta-1 found in liver homogenates was recovered in highly pure mitochondrial fractions. The functional role of TGF-beta in the mitochondrion is unknown at present.

引用

页码：467 / 477

页数：11

共 33 条

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