2 ALPHA-SUBUNITS OF THE GQ CLASS OF G-PROTEINS STIMULATE PHOSPHOINOSITIDE PHOSPHOLIPASE C-BETA-1 ACTIVITY

被引：65

作者：

TAYLOR, SJ ^{[1
]}

EXTON, JH ^{[1
]}

机构：

[1] VANDERBILT UNIV, MED CTR,SCH MED,DEPT MOLEC PHYSIOL & BIOPHYS, HOWARD HUGHES MED INST, NASHVILLE, TN 37232 USA

来源：

FEBS LETTERS | 1991年 / 286卷 / 1-2期

关键词：

G-PROTEIN; PHOSPHOINOSITIDE; PHOSPHOLIPASE-C; GQ;

D O I：

10.1016/0014-5793(91)80976-A

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two G protein alpha-subunits were detected in preparations of GTP-gamma-S-dependent, phosphoinositide-specific phospholipase C-activating proteins from bovine liver membranes. Partial resolution of the two alpha-subunits, of molecular mass 42 and 43 kDa, was achieved by Mono Q chromatography. Quantitation of the levels of each alpha-subunit and reconstitution assays demonstrated that each possessed stimulatory activity towards the beta-1 isozyme of phospholipase C. Immunoblot analysis showed that the 42 kDa protein was immunologically related to alpha-q, whereas the 43 kDa protein was related to alpha-11, another member of the G(q) class. The data thus show that two different alpha-subunits of the G(q) class of G proteins stimulate phospholipase C-beta-1 Activity.

引用

页码：214 / 216

页数：3

共 23 条

[21] EPIDERMAL GROWTH-FACTOR STIMULATES TYROSINE PHOSPHORYLATION OF PHOSPHOLIPASE C-II INDEPENDENTLY OF RECEPTOR INTERNALIZATION AND EXTRACELLULAR CALCIUM [J].