EXPRESSION OF P60V-SRC IN SACCHAROMYCES-CEREVISIAE RESULTS IN ELEVATION OF P34CDC28 KINASE-ACTIVITY AND RELEASE OF THE DEPENDENCE OF DNA-REPLICATION ON MITOSIS

被引:18
作者
BOSCHELLI, F
机构
[1] Department of Biochemistry, Wayne State University, School of Medicine, Detroit, MI 48201
关键词
D O I
10.1128/MCB.13.8.5112
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Expression of the oncogenic protein tyrosine kinase P60v-src in the yeast Saccharomyces cerevisiae has been shown to result in rapid cell death (J. S. Brugge, G. Jarosik, J. Andersen, A- Queral-Lustig, M. Fedor-Chaiken, and J. R. Broach, Mol. Cell. Biol. 7:2180-2187, 1987). Work described here demonstrates that v-Src expression results in accumulation of large-budded cells and a nuclear division block without blocking cytokinesis. Flow-cytometric analysis indicates that the DNA content of these cells is elevated beyond the G2 DNA content, and genetic studies indicate that v-Src expression causes aneuploidy. The activity of Cdc28 kinase, which controls the G1/S and G2/M transitions in S. cerevisiae, increases during galactose induction in a Src+ strain but not in an isogenic Src- strain. These observations indicate that v-Src expression disrupts p3P34CDC28 kinase regulation, allowing DNA replication to proceed in the absence of a prior mitotic event.
引用
收藏
页码:5112 / 5121
页数:10
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