BIOSYNTHETIC CONVERSION OF PHOSPHATIDYLGLYCEROL TO SN-1-SN-1' BIS(MONOACYLGLYCEROL) PHOSPHATE IN A MACROPHAGE-LIKE CELL-LINE

被引：34

作者：

AMIDON, B ^{[1
]}

SCHMITT, JD ^{[1
]}

THUREN, T ^{[1
]}

KING, L ^{[1
]}

WAITE, M ^{[1
]}

机构：

[1] WAKE FOREST UNIV,BOWMAN GRAY SCH MED,DEPT BIOCHEM,WINSTON SALEM,NC 27157

来源：

BIOCHEMISTRY | 1995年 / 34卷 / 16期

关键词：

D O I：

10.1021/bi00016a029

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bis(monoacylglycerol) phosphate has a unique stereoconfiguration of sn-glycero-1-phospho-1'-sn-glycerol and is synthesized from exogenous phosphatidylglycerol by macrophages. Previous work by our laboratory showed that the macrophage-like cell line RAW 264.7 synthesizes sn-glycero-1-phospho-1'-sn-glycerol bis(monoacylglycerol) phosphate. Here we describe studies using RAW 264.7 cells that examine the biosynthetic pathway by which bis(monoacylglycerol) phosphate is formed. Experiments were conducted using precursors that were specifically radiolabeled on the glycerol backbone in order to examine the stereoconfiguration of the intermediates and products formed in intact RAW 264.7 cells. The results of our studies indicate that a complex series of reactions are involved in the synthesis of bis(monoacylglycerol) phosphate, In this proposed pathway phosphatidylglycerol is hydrolyzed to form 1-acyllysophosphatidylglycerol which is then acylated on the headgroup, glycerol to form the sn-glycerol-phospho-1'-sn-glycerol enantiomer of bis(monoacylglycerol) phosphate, The sn-glycero-1-phospho-1'-sn-glycerol enantiomer of bis(monoacylglycerol) phosphate is then thought to undergo a stereoconversion that proceeds via the required removal of the acyl group at the sn-1 position. The resulting sn-glycerol-phospho-1'-sn-glycerol enantiomer of lysophosphatidylglycerol with the acyl moiety on the original headgroup glycerol is then acylated to form sn-glycero-1-phospho-1'-sn-glycerol bis(monoacylglycerol) phosphate.

引用

页码：5554 / 5560

页数：7

共 34 条

[11]

Franson R.C., Waite M., J. Cell Biol, 56, pp. 621-627, (1973)

[12]

Huterer S., Wherret J., J. Lipid Res, 20, pp. 966-973, (1979)

[13]

Huterer S., Wherret J.R., Can. J. Biochem, 60, pp. 599-607, (1982)

[14]

Huterer S.J., Hostetler K.Y., Gardner M.F., Wherret J.R., Biochim. Biophys. Acta, pp. 204-210, (1993)

[15]

Joutti A., Biochim. Biophys. Acta, 575, pp. 10-15, (1979)

[16]

Joutti A., Renkonen O., J. Lipid Res, 20, pp. 840-847, (1979)

[17]

Mason R.J., Stossel T.P., Vaughan M., J. Clin. Invest, 51, pp. 2399-2407, (1972)

[18]

Matsuzawa Y., Hostetler K.Y., J. Biol. Chem, 253, pp. 6650-6653, (1978)

[19]

Poorthuis B.J.H.M., Hostetler K.Y., J. Biol. Chem, 250, pp. 3297-3302, (1975)

[20]

Poorthuis B.J.H.M., Hostetler K.Y., J. Biol. Chem, 251, pp. 4596-4602, (1976)

← 1 2 3 4 →