OPTIMIZED CONDITIONS FOR ELECTROTRANSFORMATION OF BACTERIA ARE RELATED TO THE EXTENT OF ELECTROPERMEABILIZATION

被引：26

作者：

SIXOU, S ^{[1
]}

EYNARD, N ^{[1
]}

ESCOUBAS, JM ^{[1
]}

WERNER, E ^{[1
]}

TEISSIE, J ^{[1
]}

机构：

[1] CNRS,CNRS,CTR RECH BIOCHIM & GENET CELLULAIRES,118 ROUTE DE NARBONNE,F-31062 TOULOUSE,FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1088卷 / 01期

关键词：

ELECTROPERMEABILIZATION; TRANSFORMATION; ATP LEAKAGE; (SALMONELLA-TYPHIMURIUM); (ESCHERICHIA-COLI);

D O I：

10.1016/0167-4781(91)90163-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electropulsation is a simple and efficient way to introduce cloned genes into a variety of cell types, even with walled species. In the case of bacteria, we observed no direct correlation between survival rate and transformation yield. In the present work, we show that the yield of transformation is directly related to the level of the electric-field induced level of cell permeabilization. From experiments on Escherichia coli, it was confirmed that the extent of associated ATP leakage was a reliable assay. This approach was extended to other strains, such as Salmonella typhimurium, which to date had not been electrotransformed by plasmids.

引用

页码：135 / 138

页数：4

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