REPRODUCIBILITY AND QUANTITATION OF SEPARATION FOR RIBONUCLEOSIDE TRIPHOSPHATES AND DEOXYRIBONUCLEOSIDE TRIPHOSPHATES BY CAPILLARY ZONE ELECTROPHORESIS

被引：29

作者：

TAKIGIKU, R

SCHNEIDER, RE

机构：

[1] The Procter and Gamble Co., Miami Valley Laboratories, Cincinnati, OH 45239-8707

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 559卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(91)80075-R

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillary zone electrophoresis has been evaluated for the separation and quantitation of ribonucleoside and deoxyribonucleoside triphosphates. For adequate resolution, capillaries were treated to reduce electroosmotic flow and capillary zone electrophoresis was performed with negative high voltage. Results from both a fully automated and a manual instrument are found to have comparable performance characteristics. The described method is linear with a minimum concentration detection limit of approximately 0.001 mg/ml per nucleotide.

引用

页码：247 / 256

页数：10

共 37 条

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