AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR MONITORING RODENT COLONIES FOR STREPTOBACILLUS-MONILIFORMIS ANTIBODIES

被引：26

作者：

BOOT, R

BAKKER, RHG

THUIS, H

VEENEMA, JL

DEHOOG, H

机构：

[1] Department of Laboratory Animal Microbiology, Laboratory for Parasitology and Mycology, National Institute of Public Health and Environmental Protection, BA Bilthoven

来源：

LABORATORY ANIMALS | 1993年 / 27卷 / 04期

关键词：

STREPTOBACILLUS-MONILIFORMIS; SEROLOGY; ELISA; MOUSE; RAT;

D O I：

10.1258/002367793780745516

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

An enzyme-linked immunosorbent assay (ELISA) to measure Streptobacillus moniliformis antibodies in mice and rats was developed. Twelve S. moniliformis strains originating from cases of rat-bite fever and Haverhill fever in man and from various rodent species, showed considerable serological relationship. The ELISA appeared specific since antibodies to S. moniliformis were absorbed by autologous and homologous antigen, but not by heterologous bacterial antigens. Acholeplasma laidlawii showed partial serological relationship with S. moniliformis. The ELISA was validated using experimental infections in mice and rats. These studies and observations in naturally infected feral rats, confirmed that S. moniliformis is difficult to grow on primary isolation, and that the ELISA for S. moniliformis antibodies revealed more contaminated animals than culture.

引用

页码：350 / 357

页数：8

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