ACETALDEHYDE REGULATES THE GENE-EXPRESSION OF MATRIX-METALLOPROTEINASE-1 AND MATRIX-METALLOPROTEINASE-2 IN HUMAN FAT-STORING CELLS

被引：47

作者：

CASINI, A ^{[1
]}

CENI, E ^{[1
]}

SALZANO, R ^{[1
]}

MILANI, S ^{[1
]}

SCHUPPAN, D ^{[1
]}

SURRENTI, C ^{[1
]}

机构：

[1] FREE UNIV BERLIN, DEPT GASTROENTEROL, BERLIN, GERMANY

来源：

LIFE SCIENCES | 1994年 / 55卷 / 17期

关键词：

ACETALDEHYDE; FAT-STORING (ITO) CELLS; COLLAGEN; METALLOPROTEINASE; COLLAGENASE; ETHANOL;

D O I：

10.1016/0024-3205(94)00763-2

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Altered degradation of extracellular matrix has been implicated in the pathogenesis of hepatic fibrosis. We studied the effect of acetaldehyde (AcCHO) on gene expression of matrix-metalloproteinase (MMP)-1 (fibroblast type- interstitial collagenase) and MMP-2 (72 kDa gelatinase-type IV collagenase) in comparison with the AcCHO effect on collagen type I and IV synthesis in cultures of fat-storing cells (FSC) isolated from normal human livers. Cultured human FSC expressed single mRNA transcripts (2.7 and 3.2 kb) specific for MMP-1 and MMP-2, respectively. AcCHO inhibited MMP-1 mRNA levels, whereas it stimulated collagen type I mRNA and protein expression. Opposite AcCHO effects were evident on MMP-2 mRNA and collagen IV synthesis, being MMP-2 up-regulated and collagen IV down-regulated. These data suggest that regulation of MMP-1 and MMP-2 genes by AcCHO may contribute to disruption of the normal basement membrane and its replacement with fibrillar collagens in the early stages of alcoholic liver fibrosis.

引用

页码：1311 / 1316

页数：6