PROJECTION MAP OF TUBULIN IN ZINC-INDUCED SHEETS AT 4-ANGSTROM RESOLUTION

被引：31

作者：

DOWNING, KH

JONTES, J

机构：

[1] Life Sciences Division, Lawrence Berkeley Laboratory, Berkeley

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 1992年 / 109卷 / 02期

关键词：

D O I：

10.1016/1047-8477(92)90046-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tubulin polymerizes into two-dimensional, crystalline sheets in the presence of zinc ions. These sheets are well suited to structural studies by electron crystallography. We have developed conditions for forming sheets which are large and well ordered enough to provide both electron diffraction and image data to better than 4 Å resolution. In projection maps calculated from this data, the α and β monomers can be identified within the protofilaments. These results indicate that we should be able to determine the structure of tubulin in these sheets at atomic resolution. © 1992.

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页码：152 / 159

页数：8

相关论文

共 25 条

[11]

GASKIN F, 1977, J BIOL CHEM, V252, P6918

[12] MODEL FOR THE STRUCTURE OF BACTERIORHODOPSIN BASED ON HIGH-RESOLUTION ELECTRON CRYOMICROSCOPY [J].

HENDERSON, R ;

BALDWIN, JM ;

CESKA, TA ;

ZEMLIN, F ;

BECKMANN, E ;

DOWNING, KH .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 213 (04) :899-929

[13] STRUCTURE OF PURPLE MEMBRANE FROM HALOBACTERIUM-HALOBIUM - RECORDING, MEASUREMENT AND EVALUATION OF ELECTRON-MICROGRAPHS AT 3.5 A RESOLUTION [J].

HENDERSON, R ;

BALDWIN, JM ;

DOWNING, KH ;

LEPAULT, J ;

ZEMLIN, F .

ULTRAMICROSCOPY, 1986, 19 (02) :147-178

[14] QUANTITATIVE-ANALYSIS OF IMAGE-CONTRAST IN ELECTRON-MICROGRAPHS OF BEAM-SENSITIVE CRYSTALS [J].

HENDERSON, R ;

GLAESER, RM .

ULTRAMICROSCOPY, 1985, 16 (02) :139-150

[15] INDUCTION OF A SHEET POLYMER OF TUBULIN BY ZN-2+ [J].

LARSSON, H ;

WALLIN, M ;

EDSTROM, A .

EXPERIMENTAL CELL RESEARCH, 1976, 100 (01) :104-110

[16] CONFORMATIONAL STUDY OF CALF BRAIN TUBULIN [J].

LEE, JC ;

CORFMAN, D ;

FRIGON, RP ;

TIMASHEFF, SN .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1978, 185 (01) :4-14

[17]

LOWRY OH, 1951, J BIOL CHEM, V193, P265

[18] MICROTUBULE STRUCTURE STUDIED BY QUICK FREEZING - CRYOELECTRON MICROSCOPY AND FREEZE-FRACTURE [J].

MANDELKOW, EM ;

RAPP, R ;

MANDELKOW, E .

JOURNAL OF MICROSCOPY-OXFORD, 1986, 141 :361-373

[19] STRUCTURAL-CHANGES IN TUBULIN SHEETS UPON REMOVAL OF MICROTUBULE-ASSOCIATED PROTEINS [J].

MCEWEN, BF ;

CESKA, TA ;

CREPEAU, RH ;

EDELSTEIN, SJ .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 166 (02) :119-140

[20] CONSERVATION OF TUBULIN-ALPHA-BETA DIFFERENCES IN ZINC-INDUCED SHEETS WITH VARIATIONS IN PH AND MICROTUBULE-ASSOCIATED PROTEIN-CONTENT [J].

ROZYCKI, MD ;

STETZKOWSKIMARDEN, F ;

EDELSTEIN, SJ .

JOURNAL OF ULTRASTRUCTURE AND MOLECULAR STRUCTURE RESEARCH, 1988, 98 (01) :48-59