EXPRESSION OF PARATHYROID HORMONE-RELATED PEPTIDE AND ITS RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN HUMAN AMNION AND CHORION-DECIDUA - IMPLICATIONS FOR SECRETION AND FUNCTION

被引:21
作者
BRUNS, ME
FERGUSON, JE
BRUNS, DE
BURTON, DW
BRANDT, DW
JUPPNER, H
SEGRE, GV
DEFTOS, LJ
机构
[1] UNIV VIRGINIA, HLTH SCI CTR, DEPT PATHOL, BOX 214, CHARLOTTESVILLE, VA 22908 USA
[2] UNIV VIRGINIA, DEPT OBSTET & GYNECOL, CHARLOTTESVILLE, VA USA
[3] UNIV CALIF SAN DIEGO, DEPT MED, SAN DIEGO, CA 92103 USA
[4] VET ADM MED CTR, SAN DIEGO, CA 92161 USA
[5] MASSACHUSETTS GEN HOSP, DEPT MED, BOSTON, MA 02114 USA
[6] HARVARD UNIV, BOSTON, MA 02115 USA
关键词
PARATHYROID HORMONE-RELATED PROTEIN; PARATHYROID HORMONE PARATHYROID HORMONE-RELATED PEPTIDE RECEPTOR; AMNION; CHORION-DECIDUA;
D O I
10.1016/0002-9378(95)90333-X
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: Our purpose was to define the location and packaging of parathyroid hormone-related peptide in amnion-chorion and the potential target tissues for its action in fetal membranes. STUDY DESIGN: We studied fetal membranes by use of light microscopic immunocytochemistry with three monoclonal antibodies against distinct regions of the parathyroid hormone-related peptide molecule. For electron microscopy immunogold analysis with a monoclonal antibody specific to the 109-141 fragment was used to observe parathyroid hormone-related peptide intracellularly in amnion membrane and in the chorion layers. Multiplex reverse transcriptase-polymerase chain reaction with Southern blotting was used to identify parathyroid hormone/parathyroid hormone-related peptide receptor and control messenger ribonucleic acids in amnion and chorion-decidua. RESULTS: All monoclonal antibodies revealed immunoreactive parathyroid hormone-related peptide in the amniotic epithelial cells and in some fibroblast-like cells embedded in the extracellular matrix of the amnion. Parathyroid hormone-related peptide was also found in the chorion in fibroblast and trophoblast layers and in decidua. Ultrastructurally immunogold particles were evenly distributed throughout the amniotic epithelial cells and were present in apical microvilli and near the basal membranes. Electron microscopy studies of the chorion cytotrophoblast also showed freely dispersed immunogold particles of parathyroid hormone-related peptide with no packaging in secretory granules. Low to undetectable levels of parathyroid hormone/parathyroid hormone-related peptide receptor messenger ribonucleic acid were found in amnion tissue, whereas abundant receptor messenger ribonucleic acid was found in chorion-decidua. CONCLUSIONS: These results suggest the presence of a parathyroid hormone-related peptide paracrine system within the human fetal membranes.
引用
收藏
页码:739 / 746
页数:8
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