DISSOCIATION BY COOLING OF HORMONE AND CHOLERA TOXIN ACTIVATION OF ADENYLATE-CYCLASE IN INTACT-CELLS

被引:17
作者
VANSANDE, J
POCHET, R
DUMONT, JE
机构
[1] Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, B-1000 Brussels, 2, rue Evers
关键词
Adenylate cyclase; Cholera toxin; Cyclic AMP; Thyroid stimulating hormone;
D O I
10.1016/0304-4165(79)90028-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cholera toxin, through adenylate cyclase activation reproduced cyclic AMP-mediated effects of thyroid-stimulating hormone (TSH) in dog thyroid slices, i.e protein iodination, [1-14C]glucose-oxidation and hormone secretion. Iodide and carbamylcholine decreased the cyclic AMP accumulation induced by cholera toxin as well as by TSH, which supports the hypothesis of an action of these agents beyond the steps of hormone-receptor and receptor-adenylate cyclase interaction. Cooling to 20°C did not impair the TSH induced cyclic AMP accumulation in thyroid slices, but completely suppressed the cholera toxin effect. This observation has been extended to other hormones and target tissues, such as the parathyroid hormone (PTH) (kidney cortex), adrenocorticotropic hormone (ACTH) (adrenal cortex)_and luteinizing hormone (LH) (ovary systems). As in thyroid, cooling dissociated the cholera toxin and hormonal effects on cyclic AMP accumulation. In homogenate, cooling decreased cyclic AMP generation in the presence of cholera toxin but at 20°C and 16°C a cholera toxin stimulation was still observed. These results bear strongly against the hypothesis that the glycoprotein hormones TSH and LH activate adenylate cyclase by a mechanism identical to cholera toxin. © 1979.
引用
收藏
页码:282 / 292
页数:11
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