C-13 NMR METHOD FOR DETERMINATION OF THE TRANSBILAYER DISTRIBUTION OF PHOSPHATIDYLCHOLINE IN LARGE, UNILAMELLAR, PROTEIN-FREE AND PROTEIN-CONTAINING VESICLES

被引：42

作者：

GERRITSEN, WJ ^{[1
]}

VANZOELEN, EJJ ^{[1
]}

VERKLEIJ, AJ ^{[1
]}

DEKRUIJFF, B ^{[1
]}

VANDEENEN, LLM ^{[1
]}

机构：

[1] STATE UNIV UTRECHT,INST MOLEC BIOL,UTRECHT,NETHERLANDS

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 551卷 / 02期

关键词：

!sup]13[!/sup]C-NMR; Band; 3; protein; Glycophorin; Phosphatidylcholine; Transbilayer distribution;

D O I：

10.1016/0005-2736(89)90003-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. (1) Large unilamellar vesicles have been prepared from N-[Me3-13C]-18 : 1c/18 : 1c-phosphatidylcholine, both with and without the major intrinsic proteins from the human erythrocyte membrane incorporated in the bilayer. 2. (2) It is shown that the inside-outside distribution of the lipid molecules in these large unilamellar structures can be determined using 13C NMR. 3. (3) Large vesicles of 18 : 1c/18 : 1c-phosphatidylcholine containing glycophorin show an enhanced permeability to Dy3+. It is shown that the permeability barrier of these vesicles can be restored by addition of 10 mol% 18 : 1c/18 : 1c-phosphatidylethanolamine or 1-18 : 1c-lysophosphatidylcholine. © 1979.

引用

页码：248 / 259

页数：12

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