MUCOSUBSTANCES OF RABBIT GRANULOCYTES STUDIED BY MEANS OF ELECTRON-MICROSCOPIC AUTORADIOGRAPHY AND X-RAY-MICROANALYSIS

被引:15
作者
MURATA, F [1 ]
YOSHIDA, K [1 ]
OHNO, S [1 ]
NAGATA, T [1 ]
机构
[1] SHINSHU UNIV, SCH MED, DEPT ANAT, MATSUMOTO 390, JAPAN
关键词
D O I
10.1007/BF00496526
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Rabbit bone marrow cells have been studied by means of light-and electron-microscopic radioautography and X-ray microanalysis. Carrier free sulfuric acid was used as the radioactive precursor in this experiment. Immature granulocytes showed more active incorporation than mature ones. Silver grains were observed in the Golgi apparatus and granules in three kinds of granulocytes. Electron-microscopically, immature granules showed the incorporation of inorganic sulfate, while mature ones did not. Sulfur was detected in all kinds of granules of the three granulocyte types by X-ray microanalysis. It is concluded that incorporated inorganic sulfur may be utilized for the synthesis of acid glycosaminoglycans and the sulfur detected by X-ray microanalysis may be that contained in the acid glycosaminoglycan. The sulfur detected in the specific granules of the heterophil probably derives from proteins or polypeptides incorporating sulfur containing amino acids. © 1979 Springer-Verlag.
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页码:139 / 150
页数:12
相关论文
共 53 条
[21]  
LAU P, 1970, J LAB CLIN MED, V76, P739
[22]   PROBLEME DER ELEKTRONENMIKROSKOPISCHEN AUTORADIOGRAPHIE [J].
LETTRE, H ;
PAWELETZ, N .
NATURWISSENSCHAFTEN, 1966, 53 (11) :268-&
[23]   IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODS [J].
LUFT, JH .
JOURNAL OF BIOPHYSICAL AND BIOCHEMICAL CYTOLOGY, 1961, 9 (02) :409-&
[24]   CRITICAL EVALUATION OF SPECIFICITY IN ELECTRON MICROSCOPICAL RADIOAUTOGRAPHY IN ANIMAL TISSUES [J].
MONNERON, A ;
MOULE, Y .
EXPERIMENTAL CELL RESEARCH, 1969, 56 (2-3) :179-+
[25]   COEXISTENCE OF ACID-PHOSPHATASE AND ACID MUCOSUBSTANCE IN NUCLEOID OF HUMAN BLOOD-PLATELET GRANULES [J].
MURATA, F ;
HARDIN, JH ;
SPICER, SS .
HISTOCHEMIE, 1973, 35 (04) :319-329
[26]  
MURATA F, 1973, LAB INVEST, V29, P65
[27]  
MURATA F, 1975, ACTA HISTOCHEM CYTOC, V8, P76
[28]  
MURATA F, 1975, 10TH P INT C AN, P369
[29]  
MURATA F, 1977, ACTA ANAT NIPPON, V52, P44
[30]  
MURATA F, 1978, 9TH P INT C EL MICR, V2, P454