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PREPARATION AND CHARACTERIZATION OF 3 FLUORESCENT LABELS FOR PROTEINS, SUITABLE FOR STRUCTURAL STUDIES
被引:8
作者:
FORSTER, Y
HAAS, E
机构:
[1] Department of Life Science, Bar Ilan University
关键词:
D O I:
10.1006/abio.1993.1076
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
Three 7-aminocoumarin derivatives, 7-carboxymethylamino-4-trifluoromethyl-2H-1-benzopyran-2-one, 7-carboxymethylamino-4-methyl-2H-1-benzopyran-k2-one, and 7-dimethylamino-4-formyl-2H-1-benzopyran-2-one, were prepared. The reagents can be attached to primary amino groups of proteins. The labeled derivatives are stable and are characterized by a strong absorption band in the near uv (300-400 nm) and an emission band in the blue region (440-510 nm) with a large Stokes' shift. The spectral characteristics of these probes make them useful in applications of dynamic nonradiative excitation energy transfer measurements for conformational studies and analytical procedures based on simultaneous detection of labeled components in mixtures. © 1994 Academic Press, Inc. All rights reserved.
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页码:9 / 14
页数:6
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