PREPARATION OF CHEMICALLY BONDED STATIONARY PHASES IN HPLC BY AN IN-SITU PROCESS

被引：4

作者：

THIENPONT, A ^{[1
]}

FELIX, G ^{[1
]}

机构：

[1] ENSCPB,CNRS,URA 35,F-33405 TALENCE,FRANCE

来源：

CHROMATOGRAPHIA | 1993年 / 37卷 / 9-10期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; CHEMICALLY BONDED PHASES; IN-SITU BONDING; COVERAGE DENSITY;

D O I：

10.1007/BF02275788

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved in situ process for preparing chemically bonded stationary phases for HPLC direction packed silica columns has been developed. For this purpose, a modified home-made HPLC pump was used. The different parameters of the process, such as, type of silane, temperature, flow rate and concentration, have been studied to define an operational and reproducible procedure for bonding in analytical columns. In the in situ process, the sequence of silane reactivities was RSi - H >> RSi - NEt(2) greater than or equal to RSi - Cl. Stationary phases obtained are compared to those from the usual batch process.

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页码：505 / 510

页数：6

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