IMPROVED ELECTROPHORETIC METHODS FOR IDENTIFYING CEREAL VARIETIES

被引:56
作者
DUCROS, DL
WRIGLEY, CW
机构
[1] CSIRO, Wheat Research Unit, North Ryde, New South Wales
关键词
D O I
10.1002/jsfa.2740300807
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Several electrophorectic methods are compared in terms of their reliability, convenience and ability to distinguish between varieties of wheat, barley, rye and triticale using proteins extracted from the endosperm with 1M urea. Gradient gel electrophoresis offers an improvement, particularly in convenience, over the use of starch gels and uniform‐pore polyacrylamide. Varietal identification is described using gels having gradients in polyacrylamide concentration of 3‐27% or 2‐16%, both being available commercially ready for use. Electrophoresis in the latter gels at 400 V takes only half an hour for barley and an hour for wheat. In addition, a simple procedure is described for gel isoelectric focusing which, used alone or in combination with gel electrophoresis, provides high resolution of endosperm proteins for distinguishing between closely related varieties. Copyright © 1979 John Wiley & Sons, Ltd
引用
收藏
页码:785 / 794
页数:10
相关论文
共 23 条