TRANSCRIPTIONALLY ACTIVE REGIONS IN THE GENOME OF THE ARCHAEBACTERIUM HALOFERAX-VOLCANII

被引：11

作者：

TRIESELMANN, BA ^{[1
]}

CHARLEBOIS, RL ^{[1
]}

机构：

[1] UNIV OTTAWA,DEPT BIOL,30 MARIE CURIE PRIV,OTTAWA K1N 6N5,ONTARIO,CANADA

来源：

JOURNAL OF BACTERIOLOGY | 1992年 / 174卷 / 01期

关键词：

D O I：

10.1128/jb.174.1.30-34.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Transcriptionally active regions of the Haloferax volcanii genome were mapped by hybridization of radiolabeled cDNA to Southern blots of our minimal set of overlapping cosmid clones covering 96% of the 4.1-Mbp genome. Transcription during exponential growth occurred in nearly every region of the 2,920-kbp chromosome. Large parts of the 690- and the 86-kbp plasmids were transcribed, but the 440-kbp plasmid showed little expression. Transcription after a 40-min heat shock at 65-degrees-C was generally reduced, apart from a small set of strongly expressed loci all situated on the chromosome.

引用

页码：30 / 34

页数：5

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