DETECTION OF MULTIPLE VIRULENCE-ASSOCIATED GENES OF LISTERIA-MONOCYTOGENES BY PCR IN ARTIFICIALLY CONTAMINATED MILK SAMPLES

被引：59

作者：

COORAY, KJ

NISHIBORI, T

XIONG, HB

MATSUYAMA, T

FUJITA, M

MITSUYAMA, M

机构：

[1] NIIGATA UNIV, SCH MED, DEPT BACTERIOL, NIIGATA 951, JAPAN

[2] INST MED RES, DEPT FOOD & WATER BACTERIOL, COLOMBO, SRI LANKA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1994年 / 60卷 / 08期

关键词：

D O I：

10.1128/AEM.60.8.3023-3026.1994

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The inhibitory effect of milk in the PCR detection of Listeria monocytogenes could be overcome by washing the contaminated milk sample with phosphate-buffered saline and concentrating the bacteria to 1/10 of the original volume. In order to avoid a possible failure in the detection of virulent L. monocytogenes, a one-step procedure which enabled demonstration of three virulence-associated genes, prfA, hlyA, and plcB, simultaneously in a single PCR mixture was developed.

引用

页码：3023 / 3026

页数：4

共 21 条

[1] DETECTION OF LISTERIA-MONOCYTOGENES BY USING THE POLYMERASE CHAIN-REACTION
BESSESEN, MT
LUO, Q
ROTBART, HA
BLASER, MJ
ELLISON, RT
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (09) : 2930 - 2932
[2] COORDINATE REGULATION OF VIRULENCE GENES IN LISTERIA-MONOCYTOGENES REQUIRES THE PRODUCT OF THE PRFA GENE
CHAKRABORTY, T
LEIMEISTERWACHTER, M
DOMANN, E
HARTL, M
GOEBEL, W
NICHTERLEIN, T
NOTERMANS, S
[J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (02) : 568 - 574
[3] SPECIES-SPECIFIC DETECTION OF LISTERIA-MONOCYTOGENES BY DNA AMPLIFICATION
DENEER, HG
BOYCHUK, I
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (02) : 606 - 609
[4] LISTERIA-MONOCYTOGENES, A FOOD-BORNE PATHOGEN
FARBER, JM
PETERKIN, PI
[J]. MICROBIOLOGICAL REVIEWS, 1991, 55 (03) : 476 - 511
[5] PASTEURIZED MILK AS A VEHICLE OF INFECTION IN AN OUTBREAK OF LISTERIOSIS
FLEMING, DW
COCHI, SL
MACDONALD, KL
BRONDUM, J
HAYES, PS
PLIKAYTIS, BD
HOLMES, MB
AUDURIER, A
BROOME, CV
REINGOLD, AL
[J]. NEW ENGLAND JOURNAL OF MEDICINE, 1985, 312 (07) : 404 - 407
[6] DETECTION OF LISTERIA-MONOCYTOGENES IN CHEESE WITH THE MAGNETIC IMMUNO-POLYMERASE CHAIN-REACTION ASSAY
FLUIT, AC
TORENSMA, R
VISSER, MJC
AARSMAN, CJM
POPPELIER, MJJG
KELLER, BHI
KLAPWIJK, P
VERHOEF, J
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (05) : 1289 - 1293
[7] DETECTION AND IDENTIFICATION OF LISTERIA-MONOCYTOGENES IN COOKED SAUSAGE PRODUCTS AND IN MILK BY INVITRO AMPLIFICATION OF HEMOLYSIN GENE FRAGMENTS
FURRER, B
CANDRIAN, U
HOEFELEIN, C
LUETHY, J
[J]. JOURNAL OF APPLIED BACTERIOLOGY, 1991, 70 (05): : 372 - 379
[8] PURIFICATION AND CHARACTERIZATION OF AN EXTRACELLULAR 29-KILODALTON PHOSPHOLIPASE-C FROM LISTERIA-MONOCYTOGENES
GEOFFROY, C
RAVENEAU, J
BERETTI, JL
LECROISEY, A
VAZQUEZBOLAND, JA
ALOUF, JE
BERCHE, P
[J]. INFECTION AND IMMUNITY, 1991, 59 (07) : 2382 - 2388
[9] FOODBORNE LISTERIOSIS
JONES, D
[J]. LANCET, 1990, 336 (8724) : 1171 - 1174
[10] TN916-INDUCED MUTATIONS IN THE HEMOLYSIN DETERMINANT AFFECTING VIRULENCE OF LISTERIA-MONOCYTOGENES
KATHARIOU, S
METZ, P
HOF, H
GOEBEL, W
[J]. JOURNAL OF BACTERIOLOGY, 1987, 169 (03) : 1291 - 1297

← 1 2 3 →