EFFECT OF DETERGENTS ON ANTIBODY-ANTIGEN INTERACTION

被引：86

作者：

DIMITRIADIS, GJ ^{[1
]}

机构：

[1] NATL INST MED RES, RIDGEWAY, MILL HILL, LONDON NW7 1AA, ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 02期

关键词：

D O I：

10.1016/0003-2697(79)90165-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of the different detergent mixtures on immunodiffusion and immunoprecipitation was studied. The anionic detergent sodium dodecyl sulfate at concentrations above 0.2% (wt/vol) inhibits the reaction between antigen and antibody by more than 90%. Nonionic detergents at a concentration of 1% (wt/vol) have little or no detectable effect. When mixtures of various concentrations of ionic and nonionic detergent were used, the inhibitory effect of the ionic detergent decreased.

引用

页码：445 / 451

页数：7

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