A MURINE FIBROBLAST GROWTH-FACTOR (FGF) RECEPTOR EXPRESSED IN CHO CELLS IS ACTIVATED BY BASIC FGF AND KAPOSI FGF

被引：203

作者：

MANSUKHANI, A ^{[1
]}

MOSCATELLI, D ^{[1
]}

TALARICO, D ^{[1
]}

LEVYTSKA, V ^{[1
]}

BASILICO, C ^{[1
]}

机构：

[1] NYU, SCH MED, DEPT CELL BIOL, NEW YORK, NY 10016 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 11期

关键词：

fibroblast growth factor receptor phosphorylation; growth factors; mitogenic activity;

D O I：

10.1073/pnas.87.11.4378

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have cloned a murine cDNA encoding a tyrosine kinase receptor with about 90% similarity to the chicken fibroblast growth factor (FGF) receptor and the human fms-like gene (FLG) tyrosine kinase. This mouse receptor lacks 88 amino acids in the extracellular portion, leaving only two immunoglobulin-like domains compared to three in the chicken FGF receptor. The cDNA was cloned into an expression vector and transfected into receptor-negative CHO cells. We show that cells expressing the receptor can bind both basic FGF and Kaposi FGF. Although the receptor binds basic FGF with a 15- to 20-fold higher affinity, Kaposi FGF is able to induce down-regulation of the receptor to the same extent as basic FGF. The receptor is phosphorylated upon stimulation with both FGFs, DNA synthesis is stimulated, and a proliferative response is produced in cells expressing the receptor, whereas cells expressing the cDNA in the antisense orientation show none of these responses to basic FGF or Kaposi FGF. Thus this receptor can functionally interact with two growth factors of the FGF family.

引用

页码：4378 / 4382

页数：5

共 22 条

[11] NOVEL TYROSINE KINASE IDENTIFIED BY PHOSPHOTYROSINE ANTIBODY SCREENING OF CDNA LIBRARIES [J].

KORNBLUTH, S ;

PAULSON, KE ;

HANAFUSA, H .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (12) :5541-5544

[12] PURIFICATION AND COMPLEMENTARY-DNA CLONING OF A RECEPTOR FOR BASIC FIBROBLAST GROWTH-FACTOR [J].

LEE, PL ;

JOHNSON, DE ;

COUSENS, LS ;

FRIED, VA ;

WILLIAMS, LT .

SCIENCE, 1989, 245 (4913) :57-60

[13] HIGH AND LOW AFFINITY BINDING-SITES FOR BASIC FIBROBLAST GROWTH-FACTOR ON CULTURED-CELLS - ABSENCE OF A ROLE FOR LOW AFFINITY BINDING IN THE STIMULATION OF PLASMINOGEN-ACTIVATOR PRODUCTION BY BOVINE CAPILLARY ENDOTHELIAL-CELLS [J].

MOSCATELLI, D .

JOURNAL OF CELLULAR PHYSIOLOGY, 1987, 131 (01) :123-130

[14]

NEUFELD G, 1986, J BIOL CHEM, V261, P5631

[15] COMPARATIVE-STUDY OF THE TYROSINE PHOSPHORYLATION OF PROTEINS IN SWISS 3T3 FIBROBLASTS STIMULATED BY A VARIETY OF MITOGENIC AGENTS [J].

PASQUALE, EB ;

MAHER, PA ;

SINGER, SJ .

JOURNAL OF CELLULAR PHYSIOLOGY, 1988, 137 (01) :146-156

[16] 2 FORMS OF THE BASIC FIBROBLAST GROWTH-FACTOR RECEPTOR-LIKE MESSENGER-RNA ARE EXPRESSED IN THE DEVELOPING MOUSE-BRAIN [J].

REID, HH ;

WILKS, AF ;

BERNARD, O .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1596-1600

[17] RECEPTOR FOR ACIDIC FIBROBLAST GROWTH-FACTOR IS RELATED TO THE TYROSINE KINASE ENCODED BY THE FMS-LIKE GENE (FLG) [J].

RUTA, M ;

BURGESS, W ;

GIVOL, D ;

EPSTEIN, J ;

NEIGER, N ;

KAPLOW, J ;

CRUMLEY, G ;

DIONNE, C ;

JAYE, M ;

SCHLESSINGER, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) :8722-8726

[18]

RUTA M, 1988, ONCOGENE, V3, P9

[19] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[20] THE EPIDERMAL GROWTH-FACTOR RECEPTOR AS A MULTIFUNCTIONAL ALLOSTERIC PROTEIN [J].

SCHLESSINGER, J .

BIOCHEMISTRY, 1988, 27 (09) :3119-3123

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