A COMPUTER ROUTINE FOR THE SELECTION AND OPTIMIZATION OF MULTISOLVENT MOBILE PHASE SYSTEMS IN REVERSED-PHASE LIQUID-CHROMATOGRAPHY

被引：26

作者：

HEINISCH, S

RIVIERE, P

ROCCA, JL

机构：

[1] Laboratoire des Sciences Analytiques (UA CNRS 040435), Université Claude Bernard Lyon 1, Villeurbanne Cedex, 69622

来源：

CHROMATOGRAPHIA | 1991年 / 32卷 / 11-12期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; MULTISOLVENT MOBILE PHASE; OPTIMIZATION;

D O I：

10.1007/BF02327904

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A computer program is described for the optimization of reversed-phase multisolvent separations. After four initial gradient runs are carried out, calculation will locate a possible optimum with binary systems or select two binary mixtures with respect to both the analysis time and the selectivity. In this latter case, two additional experiments will locate the true optimum on a straight line joining these two binary compositions on the solvent triangle (ternary system). The efficiency of this procedure is demonstrated with an illustrative example.

引用

页码：559 / 565

页数：7

共 11 条

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