AVAILABILITY OF FLUOROGENIC REAGENTS HAVING A BENZOFURAZAN STRUCTURE IN THE BIOSCIENCES

Fluorogenic reagents having a 2,1,3-benzoxadiazole (benzofurazan) moiety are reviewed in terms of reactivity, fluorescence characteristics, sensitivity and applicability in the biosciences. Those included are 4-fluoro-7-nitrobenzofurazan (NBD-F), 4-(N,N-dimethylaminosulphonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-F), 4-aminosulphonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F), ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulphonate (SBD-F), 4-hydrazino-7-nitro-2,1,3-benzoxadiazole (NBD-H), 4-hydrazino-7-nitro-2,1,3-benzoxadiazole hydrazine (NBD-H NH2NH2), 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H), 4-aminosulphonyl-7-hydrazino-2,1,3-benzoxadiazole (ABD-H), 4-nitro-7-N-piperazino-2,1,3-benzoxadiazole (NBD-PZ), (+)-4-nitro-7-(2-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole (D-NBD-APy), (-)-4-nitro-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole (L-NBD-APy), 4-N,N-dimethylamino-7-N-piperazino-2,1,3-benzoxadiazole (DBD-PZ), 4-N,N-dimethylamino-7-N-cadaverino-2,1,3-benzoxadiazole (DBD-CD), 4-(aminosulphonyl)-7-(1-piperazynyl)-2,1,3-benzoxadiazole (ABD-PZ), 4-(aminosulphonyl)-7-(5-aminopentylamino)-2,1,3-benzoxadiazole (ABD-AP), 4-(aminosulphonyl)-7-(2-aminoethyl)2,1,3-benzoxadiazole (ABD-AE) and ammonium 7-N-piperazino-2,1,3-benzoxadiazole-4-sulphonate (SBD-P2). Their application data in liquid chromatography are listed in tables. Future trends for the benzofurazan reagents are discussed.