DETECTION OF FRANCISELLA-TULARENSIS IN BLOOD BY POLYMERASE CHAIN-REACTION

被引：72

作者：

LONG, GW

OPRANDY, JJ

NARAYANAN, RB

FORTIER, AH

PORTER, KR

NACY, CA

机构：

[1] USN,MED RES INST,INFECT DIS THREAT ASSESSMENT PROGRAM,ROCKVILLE,MD 20852

[2] WALTER REED ARMY INST RES,DEPT CELLULAR IMMUNOL,WASHINGTON,DC 20307

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1993年 / 31卷 / 01期

关键词：

D O I：

10.1128/JCM.31.1.152-154.1993

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/mul of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

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页码：152 / 154

页数：3

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