EFFICIENT REGENERATION OF BRASSICA-OLERACEA HYPOCOTYL PROTOPLASTS AND HIGH-FREQUENCY GENETIC-TRANSFORMATION BY DIRECT DNA UPTAKE

被引：15

作者：

MUKHOPADHYAY, A ^{[1
]}

TOPFER, R ^{[1
]}

PRADHAN, AK ^{[1
]}

SODHI, YS ^{[1
]}

STEINBISS, HH ^{[1
]}

SCHELL, J ^{[1
]}

PENTAL, D ^{[1
]}

机构：

[1] MAX PLANCK INST ZUCHTUNGSFORSCH,W-5000 COLOGNE,GERMANY

来源：

PLANT CELL REPORTS | 1991年 / 10卷 / 08期

关键词：

BRASSICA-OLERACEA; DIRECT GENE TRANSFER; PROTOPLAST REGENERATION; TRANSFORMATION;

D O I：

暂无

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Efficient regeneration (80%) and high frequency genetic transformation (10-33%) were achieved by culturing protoplasts isolated from hypocotyl tissues of six day old Brassica oleracea seedlings and by subjecting these protoplasts to PEG mediated direct plasmid uptake. Three different plasmid vectors carrying marker genes for resistance to methotrexate (dhfr), hygromycin (hpt) and phosphinotricin (bar) were constructed and used for transformation. Large number of normal, fertile transformants were obtained with vectors carrying hpt and bar genes. No transformants could be regenerated for resistance to methotrexate as it severely suppressed shoot differentiation.

引用

页码：375 / 379

页数：5

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