COMPARISON BETWEEN PEROXIDASE-CONJUGATED ANTIGEN OR ANTIBODY AND PEROXIDASE-ANTI-PEROXIDASE COMPLEX IN A POSTEMBEDDING PROCEDURE

被引：7

作者：

TOUGARD, C ^{[1
]}

TIXIERVIDAL, A ^{[1
]}

AVRAMEAS, S ^{[1
]}

机构：

[1] INST PASTEUR,DEPT BIOL MOLEC,UNITE IMMUNOCYTOCHIM,F-75015 PARIS,FRANCE

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 1979年 / 27卷 / 12期

关键词：

D O I：

10.1177/27.12.391996

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The staining efficiency of peroxidase labeled immunoglobulin conjugate, used either as antigen or as antibody, has been compared with that of peroxidase-anti-peroxidase complex (PAP) on ultrathin sections of araldite embedded material. The conjugate gave positive results in a two layer method as well as in a three layer method when used as antibody. No staining was observed when it was used as antigen. The conjugation seemed to impair the antigenic reactivity of immunoglobulin. The conjugate when used as antibody in the three layer method gave approximately the same staining efficiency as PAP.

引用

页码：1630 / 1633

页数：4

共 7 条

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[5] UNLABELED ANTIBODY ENZYME METHOD OF IMMUNOHISTOCHEMISTRY - PREPARATION AND PROPERTIES OF SOLUBLE ANTIGEN-ANTIBODY COMPLEX (HORSERADISH PEROXIDASE-ANTIHORSERADISH PEROXIDASE) AND ITS USE IN IDENTIFICATION OF SPIROCHETES
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[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1970, 18 (05) : 315 - &
[6] UNLABELED ANTIBODY ENZYME METHOD - ATTEMPTED USE OF PEROXIDASE-CONJUGATED ANTIGEN AS 3RD LAYER IN TECHNIQUE
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[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1977, 25 (09) : 1036 - 1042
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