THE MEASUREMENT OF ELECTRON-TRANSPORT SYSTEM ACTIVITY IN RIVER BIOFILMS

被引：127

作者：

BLENKINSOPP, SA

LOCK, MA

机构：

[1] School of Biological Sciences, University College of North Wales, Bangor

来源：

WATER RESEARCH | 1990年 / 24卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

electron transport system activity; INT; method; river biofilms;

D O I：

10.1016/0043-1354(90)90226-V

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The factors affecting the measurement of electron transport system (ETS) activity in river biofilm through the reduction of 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl tetrazolium chloride (INT) to iodonitrotetrazolium formazan (INT-formazan) have been studied. Methanol extracts INT-formazan more effectively than either propanol or ethanol. A concentration of 0.02% INT was chosen and samples should be incubated for less than 8 h. ETS activity is optimal at a circumneutral pH. ETS stimulators (NADH, NADPH and succinate) added as a check of the assay produced an increase in INT-formazan indicating that ETS activity was being measured. This assay is quick, easy to use and ideal for field studies. © 1990.

引用

页码：441 / 445

页数：5