MOLECULAR GENETIC-ANALYSIS OF DNA OBTAINED FROM FIXED, AIR DRIED OR PARAFFIN EMBEDDED SOURCES

被引：30

作者：

GRUNEWALD, K

LYONS, J

HANSENHAGGE, TE

JANSSEN, JWG

FEICHTINGER, H

BARTRAM, CR

机构：

[1] DEPT PEDIAT 2, MOLEC BIOL LAB, W-7900 ULM, GERMANY

[2] CETUS CORP, EMERYVILLE, CA 94149 USA

[3] UNIV INNSBRUCK, DEPT PATHOL, A-6020 INNSBRUCK, AUSTRIA

来源：

ANNALS OF HEMATOLOGY | 1991年 / 62卷 / 04期

关键词：

DNA; ANALYSIS; GENE REARRANGEMENT; PCR; FIXED MATERIAL;

D O I：

10.1007/BF01702923

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

In the present study methods are described for the analysis of the genomic structure of DNA isolated from tissues that have been stored up to several years as air-dried or stained bone marrow smears, bone marrow biopsies in "Histicon", chromosomal preparations in fixative or as formalin fixed tissues embedded in paraffin. By application of the new techniques clonal B- and T-cell disorders, Philadelphia chromosome translocations and ras oncogene mutations in pancreatic carcinomas could be detected. Thus, these DNA extraction procedures may open new avenues to pathological archives and enable the analysis of samples when fresh material is not available.

引用

页码：108 / 114

页数：7