SOLID-PHASE RADIOIMMUNOASSAY FOR BACTERIAL LIPOPOLYSACCHARIDE

被引：5

作者：

GUTOWSKI, JA ^{[1
]}

JACOBS, DM ^{[1
]}

机构：

[1] SUNY BUFFALO, SCH MED, DEPT MICROBIOL, BUFFALO, NY 14214 USA

来源：

IMMUNOLOGICAL COMMUNICATIONS | 1979年 / 8卷 / 03期

关键词：

D O I：

10.3109/08820137909050048

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A radioimmunoassay [RIA] for Escherichia coli O55:B5 lipopolysaccharide (LPS) is described. The LPS was derivatized by 2 new methods and subsequently radiolabeled with 125I to a specific activity of 2-4 mCi/mg, without apparent loss in its biophysical, immunological or biological activities. Using [rabbit] antibody-coated polystyrene tubes, a solid-phase RIA was developed with a sensitivity of 10-500 ng/ml of LPS.

引用

页码：347 / 364

页数：18

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