SOLID-PHASE RADIOIMMUNOASSAY FOR BACTERIAL LIPOPOLYSACCHARIDE

被引：5

作者：

GUTOWSKI, JA ^{[1
]}

JACOBS, DM ^{[1
]}

机构：

[1] SUNY BUFFALO, SCH MED, DEPT MICROBIOL, BUFFALO, NY 14214 USA

来源：

IMMUNOLOGICAL COMMUNICATIONS | 1979年 / 8卷 / 03期

关键词：

D O I：

10.3109/08820137909050048

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A radioimmunoassay [RIA] for Escherichia coli O55:B5 lipopolysaccharide (LPS) is described. The LPS was derivatized by 2 new methods and subsequently radiolabeled with 125I to a specific activity of 2-4 mCi/mg, without apparent loss in its biophysical, immunological or biological activities. Using [rabbit] antibody-coated polystyrene tubes, a solid-phase RIA was developed with a sensitivity of 10-500 ng/ml of LPS.

引用

页码：347 / 364

页数：18

共 30 条

[21] REACTION OF ENDOTOXIN AND SURFACTANTS .I. PHYSICAL AND BIOLOGICAL PROPERTIES OF ENDOTOXIN TREATED WITH SODIUM DEOXYCHOLATE [J].

RIBI, E ;

ANACKER, RL ;

BROWN, R ;

HASKINS, WT ;

MALMGREN, B ;

MILNER, KC ;

RUDBACH, JA .

JOURNAL OF BACTERIOLOGY, 1966, 92 (05) :1493-+

[22] UNE MICRO-METHODE DE LIMMUNO-ELECTROPHORESE [J].

SCHEIDEGGER, JJ .

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1955, 7 (02) :103-110

[23]

SCHRADER WH, 1963, AM J PATHOL, V42, P225

[24] A NEW METHOD OF FORMING PEPTIDE BONDS [J].

SHEEHAN, JC ;

HESS, GP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1955, 77 (04) :1067-1068

[25] TRITIATION OF ENDOTOXIN [J].

TOMASULO, PA ;

MARCH, SC ;

LEVIN, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1975, 400 (02) :399-406

[26] PREPARATION AND CHARACTERIZATION OF A RADIOIODINATED BACTERIAL LIPOPOLYSACCHARIDE [J].

ULEVITCH, RJ .

IMMUNOCHEMISTRY, 1978, 15 (03) :157-164

[27]

WARAVDEKAR VS, 1959, J BIOL CHEM, V234, P1945

[28]

Westphal O., 1965, METHODS CARBOHYDR CH, V5, P83, DOI DOI 10.1016/J.WATRES.2010.04.030

[29] BIOLOGICAL EFFECTS OF BACTERIAL ENDOTOXINS IN MAN [J].

WOLFF, SM .

JOURNAL OF INFECTIOUS DISEASES, 1973, 128 (JUL) :S259-S264

[30] SIMPLE PROCEDURE FOR LABELING LIPID-A MOIETY OF LIPOPOLYSACCHARIDE WITH I125 [J].

ZIMMERMAN, DH ;

KERN, M .

ANALYTICAL BIOCHEMISTRY, 1977, 77 (02) :340-345

← 1 2 3 →