AN NA+/K+-ATPASE INHIBITOR PROTEIN FROM RAT-BRAIN CYTOSOL

被引：9

作者：

CHANDRA, S ^{[1
]}

ADHIKARY, G ^{[1
]}

SIKDAR, R ^{[1
]}

SEN, PC ^{[1
]}

机构：

[1] BOSE INST,DEPT CHEM,CALCUTTA 700009,W BENGAL,INDIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1144卷 / 01期

关键词：

ATPASE; NA+/K+-; INHIBITOR PROTEIN; CYTOSOL; PROTEIN PURIFICATION (RAT BRAIN);

D O I：

10.1016/0005-2728(93)90027-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A protein isolated from rat brain cytosol is found to inhibit Na+/K+-ATPase in rat brain and kidney and H+/K+-ATPase from toad gastric mucosa, but has no effect on Ca2+,Mg2+-ATPase and Ca2+-ATPase isolated either from rat testis or goat spermatozoa. The inhibitor has been partially purified by ammonium sulphate precipitation followed by gel-filtration through Sephadex G-100. The inhibitor seems to bind at or close to the ATP binding site of Na+/K+-ATPase, such that the binding of the inhibitor to ATPase is reversible and competitive in nature with respect to the substrate. Optimum inhibition is observed at around the phase transition temperature of brain Na+/K+-ATPase and the inhibitory activity is only partially dependent on -SH or -NH2 group(s) of the inhibitor protein.

引用

页码：33 / 38

页数：6

共 30 条

[1] NOVEL PEPTIDE INHIBITOR (SPAI) OF NA+, K+-ATPASE FROM PORCINE INTESTINE [J].