HIGH-SPEED CHIRAL SEPARATION OF BETA-BLOCKERS BY SUPERCRITICAL-FLUID CHROMATOGRAPHY ON CHYROSINE-A

High-speed separation of beta-blockers have been successfully obtained (analysis time <2 min) using a short column packed with ChyRoSine-A CSP. Peculiar chromatographic behaviour of beta-blockers on tyrosine-derived CSPs under supercritical. conditions was evidenced by studying the curves of h versus the linear velocity u. Specific solvation of solutes by supercritical carbon dioxide is responsible for the particular conformation of the molecule of beta-blocker which enhances chiral discrimination. This was evidenced by using the reciprocality concept leading to a new CSP derived from a propranolol analogue. Thus, it was demonstrated that SFC can be used for the fast chiral separation of beta-blockers.