ROLE OF PROTEIN-KINASE-C IN THYMOCYTE APOPTOSIS INDUCED BY IRRADIATION

被引:57
作者
OJEDA, F
GUARDA, MI
MALDONADO, C
FOLCH, H
DIEHL, H
机构
[1] UNIV BREMEN, DEPT EXPTL PHYS, W-2800 BREMEN 33, GERMANY
[2] UNIV AUSTRAL CHILE, DEPT PHYS, VALDIVIA, CHILE
[3] UNIV AUSTRAL CHILE, DEPT IMMUNOL, VALDIVIA, CHILE
关键词
D O I
10.1080/09553009214551471
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The role of protein kinase C in radiation-induced death of thymocytes was studied. For this purpose murine thymocytes were irradiated and incubated for 6 h at 37-degrees-C and afterwards the fraction of fragmented DNA was measured. Results indicate that radiation-induced DNA fragmentation can be prevented by adding the protein kinase C inhibitor H-7 or staurosporine to the thymocytes during incubation time. Incubation of irradiated cells with HA-1004, an inhibitor of cAMP-dependent protein kinase, with a minor effect on protein kinase C did not affect the DNA fragmentation induced by irradiation. Incubation of cells with phorboldibutyrate gave a dose-dependent induction of DNA fragmentation. This effect can be inhibited by staurosporine. These results suggest that radiation-induced DNA fragmentation is an active cellular process in which protein kinase C plays an important role.
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页码:663 / 667
页数:5
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