GLUCOCORTICOID RECEPTOR INHIBITS MICROTUBULE ASSEMBLY IN-VITRO

被引：8

作者：

AKNER, G ^{[1
]}

WIKSTROM, AC ^{[1
]}

STROMSTEDT, PE ^{[1
]}

STOCKMAN, O ^{[1
]}

GUSTAFSSON, JK ^{[1
]}

WALLIN, M ^{[1
]}

机构：

[1] GOTHENBURG UNIV,DEPT ZOOPHYSIOL,S-41390 GOTHENBURG,SWEDEN

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1995年 / 110卷 / 1-2期

关键词：

GLUCOCORTICOID RECEPTOR; MICROTUBULES; MICROTUBULE ASSEMBLY; COLOCALIZATION;

D O I：

10.1016/0303-7207(95)03515-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effect of glucocorticoid hormones, purified glucocorticoid receptor (GR) and purified heat shock protein M(r) 90 000 (hsp90) on microtubule (MT) assembly in vitro was tested by a spectrophotometric MT assembly assay and electron microscopy. GR significantly prolonged the nucleation phase, slowed down the assembly rate and reduced the maximal amplitude of MT assembly compared with control. The effects were partially reversed by the addition of glucocorticoid hormone. GR associated with MTs. These results indicate that GR affects MT assembly in vitro, which may be a functional correlate to the structural association of GR with MTs. This implies that factors affecting GR may affect MT assembly in vivo.

引用

页码：49 / 54

页数：6

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