SPECIFIC MONITORING BY PCR AMPLIFICATION AND BIOLUMINESCENCE OF FIREFLY LUCIFERASE, GENE-TAGGED BACTERIA ADDED TO ENVIRONMENTAL-SAMPLES

被引：21

作者：

MOLLER, A

GUSTAFSSON, K

JANSSON, JK

机构：

[1] UNIV STOCKHOLM,DEPT BIOCHEM,ARRHENIUS LAB,S-10691 STOCKHOLM,SWEDEN

[2] SWEDISH NATL CHEM INSPECTORATE,S-17127 SOLNA,SWEDEN

来源：

FEMS MICROBIOLOGY ECOLOGY | 1994年 / 15卷 / 1-2期

关键词：

FIREFLY LUCIFERASE MARKER GENE; BIOLUMINESCENCE; PCR AMPLIFICATION; MICROCOSMS; GENETICALLY MODIFIED MICROORGANISMS;

D O I：

10.1016/0168-6496(94)00058-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The firefly luciferase gene, luc, was demonstrated to hold promise as a specific marker for monitoring of genetically modified bacteria in the environment. PCR amplification and bioluminescence procedures were modified and compared for environmental monitoring of luc-tagged bacteria, using Escherichia coli as a model. The methods were used to track luc-tagged bacterial cells added to intact sediment core microcosms. Detection limits for the luc-tagged cells were the following, expressed as cells per 0.5 g of sediment: 10(2), by PCR amplification; 10(3), by whole cell luminescence; and 10(3)-10(4), by measurement of luminescence in cell extracts.

引用

页码：193 / 206

页数：14

共 38 条

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