STRUCTURAL STUDIES OF THE VIBRIO-CHOLERAE O-3 O-ANTIGEN POLYSACCHARIDE

被引：37

作者：

CHOWDHURY, TA ^{[1
]}

JANSSON, PE ^{[1
]}

LINDBERG, B ^{[1
]}

LINDBERG, J ^{[1
]}

GUSTAFSSON, B ^{[1
]}

HOLME, T ^{[1
]}

机构：

[1] KAROLINSKA INST, DEPT BACTERIOL, S-10401 STOCKHOLM 60, SWEDEN

来源：

CARBOHYDRATE RESEARCH | 1991年 / 215卷 / 02期

关键词：

D O I：

10.1016/0008-6215(91)84029-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the Vibrio cholerae O:3 O-antigen polysaccharide has been investigated, mainly by n.m.r. spectroscopy, mass spectrometry, sugar and methylation analysis, and specific degradations, and is proposed to involve the following tetrasaccharide repeating-unit. [GRAPHICS] In this structure, D-D-Hep is D-glycero-D-manno-heptose, Asc is 3,6-dideoxy-L-arabino-hexose (ascarylose), and Sug is 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine) in which N-2 is acetylated and N-4 is acylated with a 3,5-dihydroxyhexanoic acid. That the 2,4-diamino-2,4,6-trideoxy-D-glucose residue is linked through O-3 and not through one of the hydroxyl groups in the 3,5-dihydroxyhexanoyl group is indicated but not definitely proved. The configuration of the latter group has not been determined. The f.a.b.-mass spectrum of the methylated O-antigen indicates that the structure given above also represents the biological repeating-unit.

引用

页码：303 / 314

页数：12

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