RELATIONSHIP BETWEEN THE PHOTOPRODUCTION OF HYDROGEN AND THE ACCUMULATION OF PHB IN NONSULFUR PURPLE BACTERIA

被引：106

作者：

HUSTEDE, E ^{[1
]}

STEINBUCHEL, A ^{[1
]}

SCHLEGEL, HG ^{[1
]}

机构：

[1] UNIV GOTTINGEN,INST MIKROBIOL,GRISEBACHSTR 8,W-3400 GOTTINGEN,GERMANY

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1993年 / 39卷 / 01期

关键词：

D O I：

10.1007/BF00166854

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Wild-type cells of Rhodobacter sphaeroides and Rhodospirillum rubrum strains Ha and S1 as well as mutant cells defective in the synthesis of poly-(3-hydroxybutyric acid) (PHB), were used to study the competition between PHB accumulation and photoproduction of hydrogen for reducing equivalents, Mutants were isolated after transposon (Tn.5) or N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. The PHB-defective mutants of R. sphaeroides lacked PHB synthase activity. In two mutants Tn5 was inserted in the PHB synthase gene. No mutants occurred that lacked the activity of beta-ketothiolase or acetoacetyl-coenzyme A reductase. Pronounced competitive effects occurred only with acetate as the organic substrate. With other organic acids or sugars, which are less readily converted to PHB than acetate, competitive effects were not significant or absent.

引用

页码：87 / 93

页数：7

共 40 条

[21] PHOTOPRODUCTION OF H-2 FROM CELLULOSE BY AN ANAEROBIC BACTERIAL CO-CULTURE [J].

ODOM, JM ;

WALL, JD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 45 (04) :1300-1305

[22] THE INFLUENCE OF POLY-BETA-HYDROXYBUTYRATE ACCUMULATION ON CELL-VOLUME AND BUOYANT DENSITY IN ALCALIGENES-EUTROPHUS [J].

PEDROSALIO, C ;

MAS, J ;

GUERRERO, R .

ARCHIVES OF MICROBIOLOGY, 1985, 143 (02) :178-184

[23] RHODOCYCLUS-PURPUREUS GEN-NOV AND SP-NOV A RING-SHAPED, VITAMIN-B12-REQUIRING MEMBER OF FAMILY RHODOSPIRILLACEAE [J].

PFENNIG, N .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1978, 28 (02) :283-288

[24]

Sambrook J., 1989, MOL CLONING LAB MANU

[25] CLONING OF THE ALCALIGENES-EUTROPHUS GENES FOR SYNTHESIS OF POLY-BETA-HYDROXYBUTYRIC ACID (PHB) AND SYNTHESIS OF PHB IN ESCHERICHIA-COLI [J].

SCHUBERT, P ;

STEINBUCHEL, A ;

SCHLEGEL, HG .

JOURNAL OF BACTERIOLOGY, 1988, 170 (12) :5837-5847

[26] CONVERSION OF ORGANIC-ACIDS TO H-2 BY RHODOSPIRILLACEAE GROWN WITH GLUTAMATE OR DINITROGEN AS NITROGEN-SOURCE [J].

SEGERS, L ;

VERSTRAETE, W .

BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (12) :2843-2853

[27] THE ROLE OF ORGANIC SUBSTRATES IN BACTERIAL PHOTOSYNTHESIS [J].

STANIER, RY ;

DOUDOROFF, M ;

KUNISAWA, R ;

CONTOPOULOU, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1959, 45 (08) :1246-1260

[28] NITROGENASE AND PHOTOSYNTHETIC ACTIVITIES OF CHEMOSTAT CULTURES OF RHODOBACTER-CAPSULATUS 37B4 GROWN UNDER DIFFERENT ILLUMINATIONS [J].

STEINBORN, B ;

OELZE, J .

ARCHIVES OF MICROBIOLOGY, 1989, 152 (01) :100-104

[29] EXCRETION OF PYRUVATE BY MUTANTS OF ALCALIGENES-EUTROPHUS, WHICH ARE IMPAIRED IN THE ACCUMULATION OF POLY(BETA-HYDROXYBUTYRIC ACID) (PHB), UNDER CONDITIONS PERMITTING SYNTHESIS OF PHB [J].

STEINBUCHEL, A ;

SCHLEGEL, HG .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1989, 31 (02) :168-175

[30]

STEINBUCHEL A, 1992, PSEUDOMONAS : MOLECULAR BIOLOGY AND BIOTECHNOLOGY, P315

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