DETECTION OF HEPATITIS-B VIRUS-DNA IN HUMAN SERUM SAMPLES - USE OF DIGOXIGENIN-LABELED OLIGONUCLEOTIDES AS MODIFIED PRIMERS FOR THE POLYMERASE CHAIN-REACTION

被引：17

作者：

ESCARCELLER, M

RODRIGUEZFRIAS, F

JARDI, R

SEGUNDO, BS

ERITJA, R

机构：

[1] UNIV AUTONOMA BARCELONA,HOSP GEN VALL HEBRON,DEPT HEPATOL,E-08035 BARCELONA,SPAIN

[2] UNIV AUTONOMA BARCELONA,HOSP GEN VALL HEBRON,DEPT BIOCHEM,E-08035 BARCELONA,SPAIN

来源：

ANALYTICAL BIOCHEMISTRY | 1992年 / 206卷 / 01期

关键词：

D O I：

10.1016/S0003-2697(05)80007-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Nonradioactive techniques have been used for the direct detection of hepatitis B virus DNA in human serum samples. A comparison of two different systems using digoxigenin-labeled DNA probes is presented. Furthermore, oligonucleotides containing one molecule of the hapten digoxigenin at the 5′-end were prepared and used as primers for the polymerase chain reaction. Amplified DNA can be directly analyzed with anti-digoxigenin Fab fragments labeled with alkaline phosphatase and chemiluminescent substrates. © 1992 Academic Press, Inc.

引用

页码：36 / 42

页数：7

共 20 条

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