SIMULTANEOUS PIXE AND PIGE ANALYSIS OF METALLOPROTEINS SEPARATED BY CELLULOSE-ACETATE ELECTROPHORESIS

被引：10

作者：

SZOKEFALVINAGY, Z ^{[1
]}

DEMETER, I ^{[1
]}

HOLLOSNAGY, K ^{[1
]}

RAISANEN, J ^{[1
]}

机构：

[1] UNIV HELSINKI,DEPT PHYS,SF-00170 HELSINKI 17,FINLAND

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 1993年 / 75卷 / 1-4期

基金：

匈牙利科学研究基金会;

关键词：

D O I：

10.1016/0168-583X(93)95635-I

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The Fe and N distributions of myoglobin bands prepared by cellulose acetate electrophoresis were simultaneously measured by detecting the proton induced Fe K X-rays and the gamma-rays of 2313 keV energy coming from the N-14(p, p'gamma)N-14 nuclear reaction at about 4 MeV proton energy. In the case of unstained bands the Fe and N distributions were very similar in shape and the integrated gamma- and X-ray yields were found to be proportional to the amounts of myoglobin supplied for electrophoresis. In the cases of stained samples no quantitative conclusions could be drawn as a consequence of the drastic denaturation of the proteins caused by the staining.

引用

页码：165 / 168

页数：4

共 7 条

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