SIMULTANEOUS PIXE AND PIGE ANALYSIS OF METALLOPROTEINS SEPARATED BY CELLULOSE-ACETATE ELECTROPHORESIS

被引:10
作者
SZOKEFALVINAGY, Z [1 ]
DEMETER, I [1 ]
HOLLOSNAGY, K [1 ]
RAISANEN, J [1 ]
机构
[1] UNIV HELSINKI,DEPT PHYS,SF-00170 HELSINKI 17,FINLAND
基金
匈牙利科学研究基金会;
关键词
D O I
10.1016/0168-583X(93)95635-I
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
The Fe and N distributions of myoglobin bands prepared by cellulose acetate electrophoresis were simultaneously measured by detecting the proton induced Fe K X-rays and the gamma-rays of 2313 keV energy coming from the N-14(p, p'gamma)N-14 nuclear reaction at about 4 MeV proton energy. In the case of unstained bands the Fe and N distributions were very similar in shape and the integrated gamma- and X-ray yields were found to be proportional to the amounts of myoglobin supplied for electrophoresis. In the cases of stained samples no quantitative conclusions could be drawn as a consequence of the drastic denaturation of the proteins caused by the staining.
引用
收藏
页码:165 / 168
页数:4
相关论文
共 7 条
[1]  
AJZENBERGSELOVE F, 1981, NUCL PHYS A, V360, P1, DOI 10.1016/0375-9474(81)90510-8
[2]  
ANDREWS AT, 1988, ELECTROPHORESIS
[3]   METAL COMPOSITION ANALYSIS OF HYDROGENASE FROM THIOCAPSA-ROSEOPERSICINA BY PROTON-INDUCED X-RAY-EMISSION SPECTROSCOPY [J].
BAGYINKA, C ;
SZOKEFALVINAGY, Z ;
DEMETER, I ;
KOVACS, KL .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 162 (01) :422-426
[4]   ELECTROPHORESIS [J].
JORGENSON, JW .
ANALYTICAL CHEMISTRY, 1986, 58 (07) :A743-&
[5]   LOCATION AND QUANTIFICATION OF METAL-IONS IN ENZYMES COMBINING POLYACRYLAMIDE-GEL ELECTROPHORESIS AND PARTICLE-INDUCED X-RAY-EMISSION [J].
SZOKEFALVINAGY, Z ;
BAGYINKA, C ;
DEMETER, I ;
KOVACS, KL ;
QUYNH, LH .
BIOLOGICAL TRACE ELEMENT RESEARCH, 1990, 26-7 :93-101
[6]   PIXE ANALYSIS OF PROTEINS SEPARATED BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].
SZOKEFALVINAGY, Z ;
DEMETER, I ;
BAGYINKA, C ;
KOVACS, KL .
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1987, 22 (1-3) :156-158
[7]   LOCATION OF PROTEIN BANDS IN CELLULOSE-ACETATE ELECTROPHORETOGRAMS MEASURING NITROGEN DISTRIBUTIONS BY THE N-14(P,P'GAMMA)N-14 NUCLEAR-REACTION [J].
SZOKEFALVINAGY, Z ;
RAISANEN, J .
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1992, 66 (1-2) :258-261